钟传奇 Zhong Chuanqi,Ph.D.

副教授

E-mail:zhongcq@xmu.edu.cn

2000/9-2004/6武汉大学生命科学学院 学士

2004/7-2009/9武汉大学生命科学学院 博士

2010/3-2016/11 厦门大学生命科学学院 博士后

2016/12至今 厦门大学生命科学学院 副教授

2000/9-2003/6School of Life Sciences, Wuhan UniversityBachelor

2004/7-2009/9School of Life Sciences, Wuhan UniversityPh.D

2010/3-2016/12School of Life Sciences, Xiamen UniversityPostdoctor

2016/12 up to nowSchool of Life Sciences, Xiamen UniversityAssociate professor

研究领域（Research Area）

我们实验室主要是利用基于SWATH-MS定量质谱来研究细胞内细胞通路。细胞中的信号传导大部分是由蛋白质复合物组装和解离来完成的。我们实验室利用定量质谱来研究细胞内源复合物的组装和解离，以期更深刻地理解信号细胞内信号传导过程。

我们首先利用亲和纯化（Affinity Purification）的方法纯化蛋白质复合物，然后用SWATH-MS质谱技术鉴定和定量蛋白质复合物中的蛋白成分。我们称之为AP-SWATH方法。此套方法不仅可以鉴定参与信号通路中的未知蛋白，也可以研究蛋白复合物中已知蛋白成分的相对比例。此外，这个方法还可以研究复合物中翻译后修饰的动态变化，比如蛋白磷酸化在信号通路中作用。我们已经利用这套方法研究了TNF和LPS信号通路。未来我们会利用这个方法研究更多的信号通路。

Our lab focus on the study of dynamic signaling pathway using SWATH-MS based quantitative proteomics. Signal transduction in cells was generally mediated by dynamic assembly and dissociation of protein complexes. To gain an in-depth insight into the signaling transduction in cells, we employed quantitative mass spectrometry to investigate assembly and dissociation of endogenous protein complexes.

We fist purified protein complexes using affinity purification, and subsequently identified and quantified protein components of protein complexes using SWATH-MS, which we referred to as AP-SWATH workflow. The AP-SWATH workflow can be utilized to identify novel proteins in the signaling pathway and investigate the stoichiometry of protein complexes. The AP-SWATH workflow has been successfully employed to study the TNF and LPS signaling pathways.

代表性论文 (Selected Publications)

代表性文章(Selected publications)（#第一作者fist author，*通讯作者corresponding author）:

1.Y. Li^#, C.Q. Zhong^#*, X. Xu, S. Cai, X. Wu, Y. Zhang, J. Chen, J. Shi, S. Lin, and J. Han* (2015). Group-DIA: analyzing multiple data-independent acquisition mass spectrometry data files. Nature methods.

2.Zhong, C. Q.^#, Y. Li^#, D. Yang, N. Zhang, X. Xu, Y. Wu, J. Chen and J. Han (2014). Quantitative phosphoproteomic analysis of RIP3-dependent protein phosphorylation in the course of TNF-induced necroptosis. Proteomics 14(6): 713-724.

3.Wu, X., L. Tian, J. Li, Y. Zhang, V. Han, Y. Li, X. Xu, H. Li, X. Chen, J. Chen, W. Jin, Y. Xie, J. Han* and C. Q. Zhong * (2012). Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11(12): 1640-1651.

其他文章

1.Han, J., C. Q. Zhong and D. W. Zhang (2011). Programmed necrosis: backup to and competitor with apoptosis in the immune system. Nat Immunol 12(12): 1143-1149.

2.Wang, X., Gong Y, Chen Z., Gong B., Xie J., Zhong, C.Q., Wang Q., Diao L., Xu, A., Han, J., Altman A. and Li Y. (2015) “TCR-induced sumoylation of the kinase PKC-0 controls T cell synapse organization and T cell activation.” Nat. Immunol. 2015 Nov;16(11).

3.Chen, W., J. Wu, L. Li, Z. Zhang, J. Ren, Y. Liang, F. Chen, C. Yang, Z. Zhou, S. S. Su, X. Zheng, Z. Zhang, C. Q. Zhong, H. Wan, M. Xiao, X. Lin, X. H. Feng and J. Han (2015). Ppm1b negatively regulates necroptosis through dephosphorylating Rip3. Nat Cell Biol 17(4): 434-444.

4.Huang, Z., S. Q. Wu, Y. Liang, X. Zhou, W. Chen, L. Li, J. Wu, Q. Zhuang, C. Chen, J. Li, C. Q. Zhong, W. Xia, R. Zhou, C. Zheng and J. Han (2015). RIP1/RIP3 binding to HSV-1 ICP6 initiates necroptosis to restrict virus propagation in mice. Cell Host Microbe 17(2): 229-242.

5.Wu, X., W. T. He, S. Tian, D. Meng, Y. Li, W. Chen, L. Li, L. Tian, C. Q. Zhong, F. Han, J. Chen and J. Han (2014). pelo is required for high efficiency viral replication. PLoS Pathog 10(4): e1004034.

6.Tian, L., J. Chen, M. Chen, C. Gui, C. Q. Zhong, L. Hong, C. Xie, X. Wu, L. Yang, V. Ahmad and J. Han (2014). The p38 pathway regulates oxidative stress tolerance by phosphorylation of mitochondrial protein IscU. J Biol Chem 289(46): 31856-31865.

7.Chen, W., Z. Zhou, L. Li, C. Q. Zhong, X. Zheng, X. Wu, Y. Zhang, H. Ma, D. Huang, W. Li, Z. Xia and J. Han (2013). Diverse sequence determinants control human and mouse receptor interacting protein 3 (RIP3) and mixed lineage kinase domain-like (MLKL) interaction in necroptotic signaling. J Biol Chem 288(23): 16247-16261.

8.Zhong, C. Q., S. Song, N. Fang, X. Liang, H. Zhu, X. F. Tang and B. Tang (2009). Improvement of low-temperature caseinolytic activity of a thermophilic subtilase by directed evolution and site-directed mutagenesis. Biotechnol Bioeng 104(5): 862-870.

9.Fang, N., C. Q. Zhong, X. Liang, X. F. Tang and B. Tang (2010). Improvement of extracellular production of a thermophilic subtilase expressed in Escherichia coli by random mutagenesis of its N-terminal propeptide. Appl Microbiol Biotechnol 85(5): 1473-1481.

10.Yang, Y. R., H. Zhu, N. Fang, X. Liang, C. Q. Zhong, X. F. Tang, P. Shen and B. Tang (2008). Cold-adapted maturation of thermophilic WF146 protease by mimicking the propeptide binding interactions of psychrophilic subtilisin S41. FEBS Lett 582(17): 2620-2626.

11.Shi, W. L., C. Q. Zhong, B. Tang and P. Shen (2007). Purification and characterization of extracellular halophilic protease from haloarchaea Natrinema sp. R6-5. Wei Sheng Wu Xue Bao 47(1): 161-163.