袁吉锋 YUAN Jifeng, Ph.D.

教授，博士生导师

闽江学者特聘教授

合成生物学、代谢工程

Synthetic Biology and Metabolic Engineering

课题组长

E-mail：jfyuan@xmu.edu.cn

西安交通大学学士（2007）；

新加坡南洋理工大学博士（2012）；

新加坡国立大学，研究科学家（2012-2016）；

新加坡科技研究局，高级研究员（2016-2017）；

新加坡国立大学，研究员（2017-2018）；

2018年至今，厦门大学生命科学学院，闽江学者特聘教授。

B.Eng., Xi’an Jiaotong University (2007);

Ph.D., Nanyang Technological University (2012);

Research Scientist, National University of Singapore (2012-2016);

Senior Research Fellow, Agency for Science, Technology and Research (2016-2017);

Research Fellow, National University of Singapore (2017-2018);

Professor, Xiamen University (2018).

研究领域

关键词：合成生物学、代谢工程、基因组编辑、生物催化与生物转化、手性不对称合成、天然产物与药物合成。

本课题组致力于研究绿色、经济、可持续的生物催化方式，以可再生的生物质为原料生产高附加值药物中间体、香精香料、精细化学品、天然产物等。通过合成生物学技术、代谢工程手段改造和设计高效细胞工厂用于生产各类高附加值产品。具体研究内容：a) 代谢路径重构技术与级联生物催化；b) 利用合成生物学理念设计动态调控系统以提高目标产物产量；c) 设计与构建高通量筛选平台定向进化拓展酶的底物专一性和立体选择性；d) 解析天然产物合成路径。

Research Area

KEY WORDS:Synthetic Biology, Metabolic Engineering, Genome Editing, Biocatalysis and Biotransformation, Asymmetric  Synthesis, Natural Product and Drug Synthesis.

Our group aims to develop green, sustainable, and cost-effective biosynthesis of high-value pharmaceutical intermediates, fragrance and flavors, fine chemicals and natural products from renewable feedstocks. By employing synthetic biology and metabolic engineering principles, our group focuses on engineering and devising efficient microbial cell factory for chemical productions. More specifically, it aims to a) develop pathway reconstruction technology and cascade biotransformation; b) dynamically regulate the pathway activity via synthetic genetic circuits; c) develop high-throughput screening approaches for directed evolution of substrate specificity and enantioselectivity of key enzymes involved in the downstream cascade biotransformation; and d) elucidate the biosynthetic pathway for natural products.

代表性论文(Selected Publications, # First author * Corresponding author)

1. Jifeng Yuan*^#, Xue Chen, Pranjul Mishra, Chi-Bun Ching. Metabolically engineered budding yeast for enhanced production of isoamyl alcohol.Applied Microbiology and Biotechnology. 2017; 101(1):465-475

2. Jifeng Yuan*^#, Chi-Bun Ching. Mitochondrial acetyl-CoA utilization pathway for terpenoid productions. Metabolic Engineering. 2016; 38:303-308

3. Jifeng Yuan*^#, Pranjul Mishra, Chi-Bun Ching. Metabolically engineered Saccharomyces cerevisiae for branched-chain ester productions. Journal of Biotechnology. 2017; 239: 90-97

4. Wen Xu, Jifeng Yuan*^#,Shuiyun Yang, Chi-Bun Ching, Jiankang Liu. Programming saposin-mediated compensatory metabolic sinks for enhancing ubiquinone productions. ACS Synth Biol. 2016; 5:1404-1411

5. Jifeng Yuan*^#, Pranjul Mishra, Chi-Bun Ching. Engineering the leucine biosynthetic pathway for isoamyl alcohol production in budding yeast. Journal of Industrial Microbiology and Biotechnology. 2016; 44(1): 107-117

6. Jifeng Yuan*^#, Chi-Bun Ching. Dynamic control of ERG9 expression for improved amorpha-4,11-diene production in Saccharomyces cerevisiae. Microbial cell factories. 2015; 14, 38.

7. Jifeng Yuan*^#, Chi-Bun Ching. Combinatorial assembly of large biochemical pathways into yeast chromosomes for improved production of value-added compounds. ACS Synth Biol. 2015;4:23-31.

8. Jifeng Yuan*^#, Chi-Bun Ching. Combinatorial engineering of mevalonate pathway for improved amorpha-4,11-diene production in budding yeast. Biotechnol Bioeng. 2014;111(3):608-17.

9. Jifeng Yuan*^#, Hongcai Gao, Jianjun Sui, Hongwei Duan, Wei Ning Chen, Chi-Bun Ching. Cytotoxicity evaluation of oxidized single-walled carbon nanotubes and graphene oxide on human hepatoma HepG2 cells: An iTRAQ-coupled 2D LC-MS/MS proteome analysis. Toxicological Sciences. 2012;126(1):149-61.

10. Jifeng Yuan*^#, Hongcai Gao, Chi-Bun Ching. Comparative protein profile of human HepG2 cells treated with graphene and single-walled carbon nanotubes: An iTRAQ-coupled 2D LC-MS/MS proteome analysis. Toxicology Letters. 2011:15; 207(3); 213-21.

11. Jifeng Yuan*^#, Hongcai Gao, Jianjun Sui, Wei Ning Chen, Chi-Bun Ching. Cytotoxicity of single-walled carbon nanotubes on human hepatoma HepG2 cells: an iTRAQ-coupled 2D LC-MS/MS proteome analysis. Toxicology In Vitro. 2011; 25(8): 1820-7.